高明霞

分析化學

教授,博士生導師

  mxgao@fudan.edu.cn

  上海市楊浦區淞滬路2005號 沐鸣平台江灣校區化學樓 A4019室

  

  http://www.hplc.fudan.edu.cn

研究興趣

  • 蛋白質組學研究是後基因組時代最前沿和最熱點的研究領域之一,其在生物組織樣品具有組成復雜🫵🏽✊、濃度動態範圍寬的特點🧖🏽‍♀️⚆。針對這一問題,多年來我們在二維高效液相色譜分離系統的研發方面開展了系列工作🤏🏿。開發了從肽段🚌、蛋白質到蛋白質復合物的大規模高通量的分離分析方法🖐🏼。並進一步發展多種復合材料對多種蛋白質的翻譯後修飾(糖基化、磷酸化👮🏼、棕櫚酰化等)進行了富集分離以及定性定量分析。

  • 研究表明循環腫瘤細胞(CTCs)被視為癌症研究的標誌物,它與癌症的擴散和轉移密切相關,發展針對CTCs的液體活檢技術對於癌症的早期診斷與治療具有決定性的意義♛。為此,我們進行了系列研究🕷,開發多種用於CTC捕獲的材料與方法,同時研發了多種高靈敏度的檢測方法(SERS、MALDI等技術)。

  • 在外泌體的分離分析方面開發的一系列研究方法👨🏼‍🚒🧑‍🦰,研究了具有不同大小的亞組。不同亞組的外泌體在細胞間交流扮演者不同的角色,了解這種尺寸的異質性有助於我們對外泌體更深層次的理解。進一步研究基於指紋圖譜的人工智能用於癌症診斷。

獲獎情況

  • 2009年度沐鸣平台復華獎教金

  • 2011榮獲沐鸣平台十佳輔導員稱號

  • 2016年,沐鸣平台優秀共產黨員

學習工作經歷

  • 2006年7月至今:沐鸣开户工作

  • 2018年1月至2019年1月,美國聖母大學,訪問學者

  • 2007年5月至12月🧑🏿‍🎄,德國🖍,慕尼黑,環境與健康國家研究中心,訪問學者

  • 2003年9月-2006年7月,沐鸣开户獲博士學位

  • 2000年9月-2003年7月,山東師範大學化學化工與材料科學學院獲碩士學位

  • 1996年9月-2000年7月,山東師範大學化學化工與材料科學學院獲學士學位

授課情況

  • 主講🏐:本科生課程《分析化學AII》🫴🏿;研究生課程《生物色譜》🕵🏻‍♀️;本科生選修課《生命中的化學元素》;

  • 參加實驗課教學:無機與分析化學實驗;高等儀器實驗

代表性論文或專著

  • Haoyang Zheng, Sheng Guan Wang, Xuantang Wang, Mingxia Gao*, Xiangmin Zhang, Deconstruction of Heterogeneity of Size-Dependent Exosome Subpopulations from Human Urine by Profiling N‑Glycoproteomics and Phosphoproteomics Simultaneously. Anal. Chem. 2020, 92, 9239−9246

  • Rongrong Jin, Jiaxi Wang, Mingxia Gao*, Xiangmin Zhang, Aminophenylboronic Acid-Functionalized Thorny-Trap-Shaped Monolayer Microarray for Efficient Capture and Release of Circulating Tumor Cells. Analytical Chemistry, 2020, 92, 3403−3408

  • Zhi Huang, Guoquan Yan, Mingxia Gao*, and Xiangmin Zhang*, Array-Based Online Two Dimensional Liquid Chromatography System Applied to Effective Depletion of High-Abundance Proteins in Human Plasma, Anal. Chem. 2016, 88, 2440-2445

  • Haoyang Zheng, Jiaxi Wang, Mingxia Gao*, Xiangmin Zhang, Titanium(IV)-functionalized zirconium-organic frameworks as dual-metal affinity probe for recognition of endogenous phosphopeptides prior to mass spectrometric quantification,Microchimica Acta, 2019, 186:829.

  • Jiaxi Wang, Jie Li, Mingxia Gao*, Xiangmin Zhang, Preparation of thickness-controlled Mg-MOFs based magnetic graphene composite as a novel hydrophilic matrix for the effective identification of glycopeptide in human urine. Nanoscale, 2019, 11, 3701 - 3709

  • Jiaxi Wang, Jie Li, Mingxia Gao**, Xiangmin Zhang*, Recent advances in covalent organic frameworks for separation and analysis of complex samples, Trends in Analytical Chemistry, 2018, 108, 98-109

  • Jiaxi Wang, Jie Li, Mingxia Gao*, Xiangmin Zhang, Self-assembling covalent organic framework functionalized magnetic graphene hydrophilic biocomposites as an ultrasensitive matrix for N-linked glycopeptide recognition, Nanoscale, 2017, 9, 10750–10756

  • Changlong Sun, Mingxia Gao*, Xiangmin Zhang, Surface-enhanced Raman scattering (SERS) imaging-guided real-time photothermal ablation of target cancer cells using polydopamine-encapsulated gold nanorods as multifunctional agents, Anal Bioanal Chem, 2017, 409:4915–4926

  • Yanan Wang, Jiaxi Wang, Mingxia Gao*, Xiangmin Zhang, Functional dual hydrophilic dendrimer-modified metal-organic framework for the selective enrichment of N-glycopeptides🆗, Proteomics, 2017, 17(10), 1700005

  • Jiaxi Wang, Jie Li, Yanan Wang, Mingxia Gao*, Xiangmin Zhang, Pengyuan Yang, Development of Versatile Metal−Organic Framework Functionalized  Magnetic Graphene Core−Shell Biocomposite for Highly Specific Recognition of Glycopeptides, ACS Appl. Mater. Interfaces 2016, 8, 27482-27489.

  • Ling Zhang, Ren Zhang, Mingxia Gao*, Xiangmin Zhang*, Facile synthesis of thiol and alkynyl contained SERS reporter molecular and its usage in assembly of polydopamine protected bioorthogonal SERS tag for live cell imaging. Talanta, 2016,158, 315-321.

  • Zhi Huang, Guoquan Yan, Mingxia Gao*, and Xiangmin Zhang*, Array-Based Online Two Dimensional Liquid Chromatography System Applied to Effective Depletion of High-Abundance Proteins in Human Plasma, Anal. Chem. 2016, 88, 2440-2445

  • Jiaxi Wang, Yanan Wang, Mingxia Gao*, Xiangmin Zhang, Pengyuan Yang, Versatile metal-organic framework- functionalized magnetic grapheme nanoporous composites: as deft matrix for high-effective extraction and purification of the N-linked glycans, Analytica Chimica Acta 2016, 932 41-48.

  • Jiaxi Wang, Jie Li, Mingxia Gao*, Xiangmin Zhang, Chunhui Deng, A novel double-component MOAC honeycomb composite with pollen grains as a template for phosphoproteomics research, Talanta, 2016, 154,141-149

  • Yanan Wang, Jiaxi Wang, Mingxia Gao*, Xiangmin Zhang*, An Ultra Hydrophilic Dendrimer-Modified Magnetic Graphene with a Polydopamine Coating for Selective Enrichment of Glycopeptides, Journal of Materials Chemistry B, 2015, 3, 8711-8716

  • Jiaxi Wang, Yanan Wang, Mingxia Gao*, Xiangmin Zhang*, Pengyuan Yang, Multilayer Hydrophilic Poly(phenol-formaldehyde resin)-Coated Magnetic Graphene for Boronic Acid Immobilization as a Novel Matrix for Glycoproteome Analysis, ACS Appl. Mater. Interfaces 2015, 7, 16011−16017

  • Changlong Sun, Ling Zhang, Ren Zhang, Mingxia Gao*, Xiangmin Zhang*, Facilely synthesized polydopamine encapsulated surface-enhanced Raman scattering (SERS) probes for multiplex tumor associated cell surface antigen detection using SERS imaging, RSC Adv., 2015, 5, 72369–72372

  • Peng Zhang, Ren Zhang, Mingxia Gao*, Xiangmin Zhang*, Novel Nitrocellulose Membrane Substrate for Efficient Analysis of Circulating Tumor Cells Coupled with Surface-Enhanced Raman Scattering Imaging, ACS Appl. Mater. Interfaces 2014, 6, 370−376

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